Rapid isolation of vitexin from leaves of kinkeliba, Combretum micranthum G. Don

Vitexin, the main flavonoid was extracted from leaves of Combretum micranthum in soxhlet with ethanol. The extract evaporated to dryness and retrieved thanks hot water. That liquid went through a degreasing again butanol. butanolic dissolved methanol, which vitexin precipitated after washing recrystallisation first obtained precipitate. From 500g dry kinkeliba leaves, series manipulations led isolation 0.0576 g vitexin, yielding 0.013% per ounce powder. degree purity 94.79% on basis HPLC/UV-Vis/MS chemical formula C21H20O10. According spectral data NMR 1H 13C molecular structure is identified.